Electrophoretic and immunochemical studies of Micrurus snake venoms.

@article{AlapeGirn1994ElectrophoreticAI,
  title={Electrophoretic and immunochemical studies of Micrurus snake venoms.},
  author={Alberto Alape-Gir{\'o}n and Bruno Lomonte and Bj{\"o}rn Gustafsson and Nelson Jorge da Silva and Monica Thelestam},
  journal={Toxicon : official journal of the International Society on Toxinology},
  year={1994},
  volume={32 6},
  pages={
          713-23
        }
}
The electrophoretic mobilities of venom components from 15 Micrurus species were studied by polyacrylamide gel electrophoresis. The venoms showed species-specific protein patterns under native (PAGE) or denaturing (urea-PAGE) conditions. However, electrophoretic patterns obtained by SDS-PAGE under reducing conditions were similar. The proteins of all venoms had mol. wts either in the range of 45 to 75 kDa or lower than 14.5 kDa. PAGE and urea-PAGE of single extraction venom samples from 22 M… 
Biological and immunochemical characterization of Micrurus altirostris venom and serum neutralization of its toxic activities.
TLDR
The data suggest that M. altirostris venom presents different biological, enzymatic and immunological characteristics from other Micrurus venoms, and some activities are not neutralized by the commercial anti-elapidic serum produced in Brazil.
Immunochemical characterization of Micrurus nigrocinctus nigrocinctus venom with monoclonal and polyclonal antibodies.
TLDR
Nigroxin showed phospholipase and myotoxic activities and appeared as a single 15 kDa band in SDS-PAGE under reducing conditions and three bands with slight differences in charge were resolved by urea-PAGES, representing isoforms named nigroxin a, b, and c.
Development of immunoassays for determination of circulating venom antigens during envenomations by coral snakes (Micrurus species).
TLDR
The assays described in this work are promising tests to estimate the severity of poisoning in envenomations by the most important coral snakes of North and Central America.
Proteomic and biological characterization of the venom of the redtail coral snake, Micrurus mipartitus (Elapidae), from Colombia and Costa Rica.
TLDR
Venomics of Micrurus species may provide a valuable platform for the rational design of immunizing cocktails to obtain polyspecific antivenoms for this highly diverse group of American elapids.
Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis.
TLDR
Results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice, and most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity.
Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae).
TLDR
The proteome of the venom of Micrurus nigrocinctus (Central American coral snake) was analyzed by a "venomics" approach, revealing a complex protein composition that mainly exert myotoxicity.
Characterization of α‐Neurotoxin and Phospholipase A2 Activities from Micrurus Venoms
New World elapids are coral snakes that belong to the genus Micrurus, and for which the venom biochemistry is mostly unknown. Analysis has been difficult because the coral snakes produce small
Diversity of Micrurus Snake Species Related to Their Venom Toxic Effects and the Prospective of Antivenom Neutralization
TLDR
The data show that the antivenom used for human therapy in Brazil is not fully able to neutralize the main toxic activities present in the venoms from all Micrurus species occurring in the country, probably reflecting the adaptation of the snakes from this genus to vastly dissimilar habitats.
Recognition of venom fractions from Brazilian coral snakes by antielapid serum
TLDR
The results suggest restricted efficacy of the antielapid serum, due to its limitations against species from the Amazon region, reinforcing the need for a review of intraspecific and interspecific studies of Micrurus venoms.
Venoms of Micrurus coral snakes: Evolutionary trends in compositional patterns emerging from proteomic analyses.
TLDR
Results gathered to date reveal a dichotomy of venom phenotypes regarding the relative abundance of the omnipresent phospholipases A2 and 'three-finger' toxins within Micrurus, and two divergent toxin expression phenotypes appear to be related to phylogenetic positions and geographical distributions along a North-South axis in the Americas.
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References

SHOWING 1-10 OF 32 REFERENCES
A comparative study on the electrophoretic patterns of snake venoms.
  • N. Tan, G. Ponnudurai
  • Biology, Medicine
    Comparative biochemistry and physiology. B, Comparative biochemistry
  • 1992
1. Examination of the polyacrylamide gel electrophoretic (PAGE) and SDS-PAGE patterns of snake venoms shows that these patterns are useful for species differentiation (and hence identification) for
The biological properties of venoms of some American coral snakes (Genus micrurus).
  • N. Tan, G. Ponnudurai
  • Biology, Medicine
    Comparative biochemistry and physiology. B, Comparative biochemistry
  • 1992
TLDR
The Micrurus venoms appear to exhibit biological properties similar to other elapid venoms found in Asia and Africa, however, there are no common characteristics in the biological properties of the venoms examined at the generic level.
Immunochemical characterization of Micrurus nigrocinctus nigrocinctus venom with monoclonal and polyclonal antibodies.
TLDR
Nigroxin showed phospholipase and myotoxic activities and appeared as a single 15 kDa band in SDS-PAGE under reducing conditions and three bands with slight differences in charge were resolved by urea-PAGES, representing isoforms named nigroxin a, b, and c.
Comparative chromatography of Brazilian coral snake (Micrurus) venoms.
TLDR
Venoms of M. rondonianus and M. surinamensis are shown to be significantly different in their peptide composition from other Micrurus venoms.
Immunological and histochemical identity of esterases and other antigens in elapid venoms.
  • D. Munjal, W. Elliott
  • Biology, Medicine
    Toxicon : official journal of the International Society on Toxinology
  • 1972
TLDR
Antibodies were prepared by hyperimmunizing rabbits with Naja naja, Bungarus fasciatus, Agkistrodon piscivorus and Apis mellifera (honeybee) venoms and it has been shown that several of the esterases present in Naja species are immunologically identical.
Common Antigens in Snake Venoms
TLDR
Soon after Sewall's (1887) demonstration of the immunogenic properties of snake venom and the development of bacterial antitoxins, therapeutic antisera to snake venoms were developed on a more or less empiric basis with imperfect understanding of the nature and complexity of the venoms.
Coral snake venoms. In vitro relation of neutralizing and precipitating antibodies.
Abstract Cross neutralization and precipitin tests were performed with coral snake venoms and rabbit antisera against five venoms to determine the most suitable venoms for producing a polyvalent
Comparative enzymatic composition of Brazilian coral snake (Micrurus) venoms.
  • S. Aird, N. J. da Silva
  • Biology, Medicine
    Comparative biochemistry and physiology. B, Comparative biochemistry
  • 1991
TLDR
Venom of M. surinamensis was significantly different from those of its congeners in most assays, and protease activity against synthetic or dyed natural substrates was generally negligible in all elapid venoms examined.
Variation in venom constituents within a single isolated population of peninsula tiger snake (Notechis ater niger).
  • V. Williams, J. White
  • Biology, Medicine
    Toxicon : official journal of the International Society on Toxinology
  • 1987
TLDR
Venom from seven individuals in a homogeneous isolated population of Notechis ater niger on Roxby Island was subjected to gel filtration liquid chromatography and polyacrylamide gel electrophoresis to determine the extent of homogeneity of the venom constituent profile.
Skeletal muscle necrosis induced by a phospholipase A2 isolated from the venom of the coral snake Micrurus nigrocinctus nigrocinctus.
1. The venom of the coral snake Micrurus nigrocinctus nigrocinctus was fractionated by reverse-phase high performance liquid chromatography and an acidic myotoxic phospholipase A2 was purified to
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