Electrophoretic analysis of ruminal degradability of corn proteins.

  title={Electrophoretic analysis of ruminal degradability of corn proteins.},
  author={Donato Romagnolo and C E Polan and William E. Barbeau},
  journal={Journal of dairy science},
  volume={77 4},
Albumin, globulin, and prolamin fractions were extracted from corn meal in water, .5 M NaCl, and 50% (vol/vol) 1-propanol and examined by SDS-PAGE and densitometric scanning to investigate fractional degradation rates of corn proteins. Several protein fractions were identified for globulins, zein prolamins, and glutelins. Ruminal degradability of individual subfractions was evaluated by suspension of corn and corn gluten meal samples in the rumen of lactating dairy cows from 0 to 72 h… CONTINUE READING