Electrophoretic analysis of a gastric cancer-associated acid proteinase using a highly sensitive detection system.

Abstract

A highly sensitive detection system for acid proteinase separated on polyacrylamide gel was established. This system consisted of two-dimensional electrophoresis, combined with isoelectric focusing and polyacrylamide gel electrophoresis, and casein clotting (caseogram). Human urine, serum and gastric tissues obtained from normal individuals and gastric… (More)

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