This research paper examined the impact of e-commerce on business performance with particular reference to the selected supermarkets in Ibadan metropolis. The study sample was made up of 8 Supermarkets. Structured questionnaire designed by the researchers were used to collect data from each operator and 5 staff respectively, totaling 48 respondents. Data analysis was conducted with simple regression analysis. The result showed that e-commerce adoption has significant impact on service operations, cost operation reductions and profit levels. It was concluded that adoption of e-commerce by Nigerian supermarkets will reduce transaction cost, improve service operations, expand business base, better understand the needs of foreign customers, and increase profit levels. The paper recommended that the operators and staff of supermarkets should embark on more effective Information Technology (IT) training in order to further enhance their performance. And also management of supermarkets should procure quality IT gadgets that will enhance efficiency and customers’ retention.