Electron transfer kinetics in purified reaction centers from the green sulfur bacterium Chlorobium tepidum studied by multiple-flash excitation.

@article{Kusumoto1999ElectronTK,
  title={Electron transfer kinetics in purified reaction centers from the green sulfur bacterium Chlorobium tepidum studied by multiple-flash excitation.},
  author={Noriaki Kusumoto and Pierre S{\'e}tif and Klaus Brettel and Daisuke Seo and Hiromu Sakurai},
  journal={Biochemistry},
  year={1999},
  volume={38 37},
  pages={12124-37}
}
Reaction center preparations from the green sulfur bacterium Chlorobium tepidum, which contain monoheme cytochrome c, were studied by flash-absorption spectroscopy in the near-UV, visible, and near-infrared regions. The decay kinetics of the photooxidized primary donor P840(+), together with the amount of photooxidized cytochrome c, were analyzed along a series of four flashes spaced by 1 ms: 95% of the P840(+) was reduced by cytochrome c with a t(1/2) of approximately 65 micros after the first… CONTINUE READING

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