Electron tomography of nascent herpes simplex virus virions.

  title={Electron tomography of nascent herpes simplex virus virions.},
  author={Joel D. Baines and Chyong-ere Hsieh and Elizabeth G Wills and Carmen A. Mannella and M. Marko},
  journal={Journal of virology},
  volume={81 6},
Cells infected with herpes simplex virus type 1 (HSV-1) were conventionally embedded or freeze substituted after high-pressure freezing and stained with uranyl acetate. Electron tomograms of capsids attached to or undergoing envelopment at the inner nuclear membrane (INM), capsids within cytoplasmic vesicles near the nuclear membrane, and extracellular virions revealed the following phenomena. (i) Nucleocapsids undergoing envelopment at the INM, or B capsids abutting the INM, were connected to… CONTINUE READING