Electron-tomographic analysis of intraflagellar transport particle trains in situ

@article{Pigino2009ElectrontomographicAO,
  title={Electron-tomographic analysis of intraflagellar transport particle trains in situ},
  author={G. Pigino and S. Geimer and S. Lanzavecchia and E. Paccagnini and F. Cantele and D. Diener and J. Rosenbaum and P. Lupetti},
  journal={The Journal of Cell Biology},
  year={2009},
  volume={187},
  pages={135 - 148}
}
Ultrastructural study of Chlamydomonas cilia shows that anterograde IFT particles form trains that are long and narrow, while retrograde IFT form short, compact particle trains. 
Cilia Train Spotting.
TLDR
Reporting in Science, Stepanek and Pigino (2016) employ correlative light and electron microscopy in algae to determine the ultrastructure of anterograde and retrograde trains and discover that these trains avoid collision by running on B- and A-tubules, respectively. Expand
Cryoelectron tomography of radial spokes in cilia and flagella
Cryo-EM tomography of wild-type and mutant cilia and flagella from Tetrahymena and Chlamydomonas reveals new information on the substructure of radial spokes.
Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model
Chlamydomonas reinhardtii IFT particle trains, important for flagella maintenance and assembly, are observed to decrease in size as a function of cilia length.
The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function
An inducible dynein heavy chain 1b mutant reveals that robust retrograde intraflagellar transport is required for flagellar assembly and function but not the maintenance of flagellar length.
Control of vertebrate intraflagellar transport by the planar cell polarity effector Fuz
The planar cell polarity effector Fuz is required for normal particle dynamics of the intraflagellar transport system, specifically in the retrograde transport of proteins.
Intraflagellar transport proteins in ciliogenesis of photoreceptor cells
TLDR
The involvement of IFT proteins in the ciliogenesis of mammalian photoreceptor cilia is analysed and it is shown that these proteins play a central role in the assembly and maintenance of cilia. Expand
Flagella and Cilia: The Long and the Short of It
Intraflagellar transport is essential for the assembly and function of cilia and flagella, and recent work shows that intraflagellar transport complexes - or trains - fall into two classes accordingExpand
Intraflagellar transport molecules in ciliary and nonciliary cells of the retina
IFT proteins are differentially localized in photoreceptor cilia, including within the inner segment, and some are shown to function in trafficking in nonciliated retinal neurons.
[Elongation of the axoneme and dynamics of intraflagellar transport].
TLDR
The mode of assembly of these complex organelles that depends on a dynamic process called intraflagellar transport or IFT is discussed, which delivers structural elements at the distal end of the cilium where assembly takes place, thereby allowing the growth of the organelle. Expand
Electron tomography of IFT particles.
TLDR
This chapter describes and discusses the strategy to produce the first 3D models of in situ IFT trains in flat-embedded flagella, and provides detailed information about the acquisition of tomographic images, the simultaneous alignment of the double-tilt tomographic series, and the analysis of the tomograms by subtomogram averaging for the generation of detailed 3D model of IFT particles. Expand
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The flagellum protrudes from the cell surface extending tens or even hundreds of microns into the external medium, which must import all the macromolecules required for its assembly, maintenance, and function. Expand
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Cryo-electron tomography is used to investigate the 3D structure and macromolecular organization of intact, frozen-hydrated sea urchin sperm flagella in a quiescent state and determines that the heptameric rings of the motor domains in the outer dynein arm complex lie parallel to the plane that contains the axes of neighboring flagellar microtubules. Expand
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We have used cryo-electron tomography to investigate the 3D structure and macromolecular organization of intact, frozenhydrated sea urchin sperm flagella in a quiescent state. The tomographicExpand
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TLDR
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TLDR
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TLDR
The occurrence of the same set of phenotypes, as the result of a mutation in any one of three loci, suggests the hypothesis that complex A is a portion of the IFT particles specifically involved in retrograde intraflagellar movement. Expand
Dissecting the Molecular Mechanisms of Intraflagellar Transport in Chlamydomonas
TLDR
A model for IFT in which tip turnaround involves dissociation of IFT complexes A and B and release of inactive cDynein1b from complex B is supported and the IFT tip turnaround point most likely is localized distal to the plus end of the outer-doublet B MTs. Expand
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