Electron-spin-resonance evidence for enzymic reduction of oxygen to a free radical, the superoxide ion.

  title={Electron-spin-resonance evidence for enzymic reduction of oxygen to a free radical, the superoxide ion.},
  author={Peter Knowles and John F. Gibson and F M Pick and Robert C. Bray},
  journal={The Biochemical journal},
  volume={111 1},
1. An electron-spin-resonance signal with g( parallel)2.08 and g( perpendicular)2.00 is observed by the rapid-freezing technique during the oxidation of substrates by molecular oxygen catalysed by xanthine oxidase at pH10. 2. The intensity of this signal is shown to depend on oxygen rather than on enzyme concentration, indicating that it is due to an oxygen free radical and not to the enzyme. 3. The same species is shown to be produced in the reaction at pH10 between hydrogen peroxide and… CONTINUE READING


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