Electron paramagnetic resonance oximetry as a quantitative method to measure cellular respiration: a consideration of oxygen diffusion interference.

@article{Presley2006ElectronPR,
  title={Electron paramagnetic resonance oximetry as a quantitative method to measure cellular respiration: a consideration of oxygen diffusion interference.},
  author={Tennille D Presley and Periannan Kuppusamy and Jay L. Zweier and Govindasamy Ilangovan},
  journal={Biophysical journal},
  year={2006},
  volume={91 12},
  pages={4623-31}
}
Electron paramagnetic resonance (EPR) oximetry is being widely used to measure the oxygen consumption of cells, mitochondria, and submitochondrial particles. However, further improvement of this technique, in terms of data analysis, is required to use it as a quantitative tool. Here, we present a new approach for quantitative analysis of cellular respiration using EPR oximetry. The course of oxygen consumption by cells in suspension has been observed to have three distinct zones: pO(2… CONTINUE READING