Electron paramagnetic resonance analysis of nitrosylhemoglobin in humans during NO inhalation.

@article{Piknova2005ElectronPR,
  title={Electron paramagnetic resonance analysis of nitrosylhemoglobin in humans during NO inhalation.},
  author={Barbora Piknova and Mark T Gladwin and Alan N Schechter and Neil Hogg},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 49},
  pages={40583-8}
}
The reactions of nitric oxide with hemoglobin play an important role in explaining the vascular biology of this free radical. It is perhaps surprising that the level of nitrosylhemoglobin (HbNO) in which NO is bound to the ferrous hemoglobin heme in whole human blood under basal and stimulated conditions is a matter of some controversy, with measurements ranging from <1 nm to close to 10 mum. In order to examine HbNO levels in human blood by using EPR spectroscopy, we have developed a… CONTINUE READING