Electron microscopy of vitrified-hydrated La Crosse virus.

  title={Electron microscopy of vitrified-hydrated La Crosse virus.},
  author={Yeshayahu Talmon and B. V. Venkataram Prasad and J P Clerx and Guo Jin Wang and Wah Chiu and Martinez J. Hewlett},
  journal={Journal of virology},
  volume={61 7},
La Crosse (LAC) virions were cryopreserved by rapid freezing in a thin layer of vitreous ice. The vitrified-hydrated LAC virions were subsequently imaged at -170 degrees C in a transmission electron microscope equipped with a low-temperature specimen holder. This cryoelectron microscopic technique eliminates the artifacts frequently associated with negative staining. Images of vitrified-hydrated LAC virions clearly revealed surface spikes as well as bilayer structure. Size measurements of the… CONTINUE READING
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