Electron microscopy of spread maize pachytene synaptonemal complexes

@article{Gillies2004ElectronMO,
  title={Electron microscopy of spread maize pachytene synaptonemal complexes},
  author={C. B. Gillies},
  journal={Chromosoma},
  year={2004},
  volume={83},
  pages={575-591}
}
The Counce-Meyer microspreading technique for animal synaptonemal complexes (SCs) has been adapted to allow spreading of the SCs of maize pachytene microsporocytes for examination in the electron microscope (EM). The spread nuclei were well dispersed and flattened, and unstained SCs could be seen with light microscope (LM) phase optics. After PTA or ammoniacal silver staining, the SCs and kinetochores were readily recognized in the EM. Variable degrees of asynapsis, stretching of the SCs, and… CONTINUE READING
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