Electron microscopy of spermatocytes previously studied in life: methods and some observations on micromanipulated chromosomes.

@article{Nicklas1979ElectronMO,
  title={Electron microscopy of spermatocytes previously studied in life: methods and some observations on micromanipulated chromosomes.},
  author={R. Bruce Nicklas and Bill R. Brinkley and Daniel A. Pepper and Donna F. Kubai and Geoffrey K. Rickards},
  journal={Journal of cell science},
  year={1979},
  volume={35},
  pages={87-104}
}
A new method is offered for combined living cell and electron-microscopic studies of spermatocytes (or other cells) which normally do not adhere to glass. The key step is micro-injection of glutaraldehyde near the target cell whenever desired during observation in life. Fixation begins and simultaneously the cell is stuck very firmly to the underlying coverslip. The method is easy and reliable: cells are almost never lost and are well preserved, except for membranes. The application of the… CONTINUE READING
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1 Excerpt

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