Electron microscopy of mammalian cells in the absence of fixing, freezing, dehydration, or specimen coating.

@article{Stokes2003ElectronMO,
  title={Electron microscopy of mammalian cells in the absence of fixing, freezing, dehydration, or specimen coating.},
  author={Debbie J. Stokes and Susan M Rea and Serena M. Best and William Bonfield},
  journal={Scanning},
  year={2003},
  volume={25 4},
  pages={181-4}
}
Human osteoblast-like (bone-forming) cells were imaged using environmental scanning electron microscopy (ESEM). The cells were hydrated, unfrozen, and uncoated. Specimens were cooled to 3 degrees C and imaged in water vapor, with partial pressures varying from saturated conditions to a humidity of approximately 50%, relative to pure water. The ESEM images show the presence of cell nuclei, nucleoli, and cytoplasmic membranes. Comparisons between chemically fixed and unfixed specimens (neither… CONTINUE READING