Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples.

@article{Mcdowall1983ElectronMO,
  title={Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples.},
  author={A W Mcdowall and James J Chang and Roy Freeman and Jean Lepault and Christi A. Walter and Jacques Dubochet},
  journal={Journal of microscopy},
  year={1983},
  volume={131 Pt 1},
  pages={1-9}
}
The preparation and high resolution observation of frozen hydrated thin sections has been studied by transmission electron microscopy (TEM and STEM) on model systems, including pure water, protein solutions, catalase crystals, myelin sheath and various tissues. The state of the ice is determined by electron diffraction. Mass measurement in the electron microscope is used to determine section thickness and control hydration. An adequate depth of vitrified material for sectioning can be obtained… CONTINUE READING

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