Electron microscopy of cardiac 3D nanodynamics: form, function, future

@article{Kohl2022ElectronMO,
  title={Electron microscopy of cardiac 3D nanodynamics: form, function, future},
  author={Peter Kohl and Joachim Greiner and Eva A. Rog-Zielinska},
  journal={Nature Reviews Cardiology},
  year={2022},
  pages={1 - 13}
}
The 3D nanostructure of the heart, its dynamic deformation during cycles of contraction and relaxation, and the effects of this deformation on cell function remain largely uncharted territory. Over the past decade, the first inroads have been made towards 3D reconstruction of heart cells, with a native resolution of around 1 nm3, and of individual molecules relevant to heart function at a near-atomic scale. These advances have provided access to a new generation of data and have driven the… 

References

SHOWING 1-10 OF 165 REFERENCES
Superresolution microscopy in heart - cardiac nanoscopy.
Applications of electron cryo-microscopy to cardiovascular research.
TLDR
This chapter describes approaches for identifying the extracellular and intracellular domains of the 3D structure of the L-type voltage-gated calcium channel and also incorporates general details for labeling and visualizing His-tagged proteins.
Mitochondrial Deformation During the Cardiac Mechanical Cycle
TLDR
Three‐dimensional ultrastructural evidence is provided for mechanically induced mitochondrial deformation in rabbit ventricular cardiomyocytes over a range of sarcomere lengths representing myocardial tissue stretch, an unloaded “slack” state, and contracture to provide a structural framework that may aide interpretation of mechanically‐regulated molecular signaling in cardiac cells.
Cryo-electron tomography of cardiac myofibrils reveals a 3D lattice spring within the Z-discs
TLDR
3D cryo-electron tomography structures of Z-disc from porcine cardiac myofibrils in relaxed and activated states show that α-actinin dimers exhibit contraction dependent swinging and sliding motions in response to a global twist in the F-Actin lattice.
Three‐dimensional electron microscopy techniques for unravelling mitochondrial dysfunction in heart failure and identification of new pharmacological targets
TLDR
3‐D EM techniques have entered a new era of structural biology and are poised to play a pivotal role in discovering new therapies targeting mitochondria for treating heart failure.
Three-Dimensional Reconstruction of Cardiac Sarcoplasmic Reticulum Reveals a Continuous Network Linking Transverse-Tubules: This Organization Is Perturbed in Heart Failure
TLDR
The distribution of the SR within the cell is related to interspecies differences in excitation–contraction coupling, and the first detailed analysis of SR remodeling as a result of heart failure is reported.
A guide to the 3D structure of the ryanodine receptor type 1 by cryoEM
  • M. Samsó
  • Biology
    Protein science : a publication of the Protein Society
  • 2017
TLDR
The first near‐atomic structures of the ryanodine receptor reveal a complex orchestration of domains controlling the channel's function, and help to understand how this could break down as a consequence of disease‐causing mutations.
...
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