Electron microscopy of bacterial ribosomal subunits


Ribosomal subunits have been purified by centrifugation from a crude extract in a 5 to 2070 linear sucrose gradient. The crude extract and the sucrose solutions were prepared in 0.1 rnM magnesium acetate and 5 mM Tris-HCI, pH 7.2 (Tris buffer). As observed with different electron microscopic techniques, the 50S ribosomal subunit was often found to possess a… (More)
DOI: 10.1007/BF02219136