Electron microscopy of amplified DNA forms in antifolate-resistant Leishmania.

@article{Hightower1987ElectronMO,
  title={Electron microscopy of amplified DNA forms in antifolate-resistant Leishmania.},
  author={Robin C. Hightower and Mei Lie Wong and Luis Miguel Ru{\'i}z-P{\'e}rez and Daniel V Santi},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 30},
  pages={
          14618-24
        }
}
Three independently derived antifolate-resistant Leishmania major cell lines overproduce the bifunctional protein thymidylate synthase-dihydrofolate reductase (TS-DHFR) by amplification of a region of DNA (R-region DNA) that contains the gene for TS-DHFR. On orthogonal-field-alteration gel electrophoresis (OFAGE), the extrachromosomal R-region DNAs are circular molecules, and different forms of R-region DNA within these cell lines are resolved. The R-region DNAs migrate aberrantly on OFAGE with… CONTINUE READING