Electron microscopy and biochemical analysis of mouse metaphase chromosomes after digestion with restriction endonucleases

@article{Goslvez1990ElectronMA,
  title={Electron microscopy and biochemical analysis of mouse metaphase chromosomes after digestion with restriction endonucleases},
  author={Jaime Gos{\'a}lvez and A. T. Sumner and Carmen L{\'o}pez-Fern{\'a}ndez and R. Rossino and V. Goyanes and Roberto Mezzanotte},
  journal={Chromosoma},
  year={1990},
  volume={99},
  pages={36-43}
}
Electron microscopy (EM) of whole mounted mouse chromosomes, light microscopy (LM), and agarose gel electrophoresis of DNA were used to investigate the cytological effect on chromosomes of digestion with the restriction endonucleases (REs) AluI, HinfI, HaeIII and HpaII. Treatment with AluI produces C-banding as seen by LM, cuts DNA into small fragments, and reduces the density of centromeres and disperses the chromatin of the arms as determined by EM. Treatment with HinfI produces C-banding… CONTINUE READING

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