Electron microscopical and histochemical studies of differentiation and function of the cephalopod gill (Sepia officinalis L.)

  title={Electron microscopical and histochemical studies of differentiation and function of the cephalopod gill (Sepia officinalis L.)},
  author={Rudolf Schipp and Stephan Mollenhauer and Sigurd von Boletzky},
SummaryPhase contrast and electron microscopical investigations on the gill ofSepia officinalis L., from the stage shortly before hatching to the adult stage, demonstrate (1) peripheral respiratory epithelial areas and (2) highly folded epithelia rich in mitochondria in the concave recesses of the gill lamellae. Enzyme-histochemical and cytochemical findings, and in particular the GOT proof, suggest by analogy with the chloride cells of the teleosts that the cells of this second type of… 

Light- and electron microscopical studies on the anatomy and function of the gills of krill (Euphausiacea, Crustacea)

Fine-structural observations revealed several cell types in the euphausiid gills, considered from their morphological characteristics to be transport cells, respiratory cells, secretory cells serving a transepithelial ion transport, gas exchange and secretion, and a further cell type presumably has a regulatory and/or filtering function on the haemolymphic current.

Ultrastructural Studies on the Epidermis of Adult Octopus Vulgaris Cuvier, 1797

  • S. Lenz
  • Biology, Environmental Science
  • 1999
The epidermis of the ventral funnel and the mantle of adult Octopus vulgaris Cuvier, 1797 consists of a single layer of cells that rests on a basal lamina and distinct cell types named: columnar cells, glial cells, tuft cells, and gland cells.

Histological Studies on Tissues of Loligo duvauceli (Orbigny, 1848) in Pazhayar coastal water, Nagapattinam District Tamilnadu

The aim of this work was to observe the structural and functional integration of the different organs like muscle, gill, liver and gonad of Loligo.

Localization of ion-regulatory epithelia in embryos and hatchlings of two cephalopods

The tissue distribution and ontogeny of Na+/K+-ATPase has been examined as an indicator for ion-regulatory epithelia in whole animal sections of embryos and hatchlings of two cephalopod species and suggests a higher sensitivity of squid vs. cuttlefish embryos to environmental acid-base disturbances.

Tissues of Paralarvae and Juvenile Cephalopods

A detailed view of the anatomy of premature hatchlings of the squid and the cuttlefish and of paralarvae of O. vulgaris is provided and this is organized into sections of the functional “ systems” (e.g. respiratory system, excretory system).

Ontogenetic and phylogenetic aspects of the cephalopod circulatory system

A preliminary comparison with the yolk sac of Nautilus is made, which allows one to assess the relative importance of embryo size and bauplan constraints, respectively.

A study of the electrical polarization of Sepia officinalis yolk envelope, a role for Na+/K+-ATPases in osmoregulation?

Electrophysiological recordings and immunostaining showed that the chorion is only a passive barrier for ions, that a complex transepithelial potential difference occurs through the yolk epithelium, and that ionocyte-like cells and Na+/K+-ATPase activity could participate to this yolk polarization.

New insights into ion regulation of cephalopod molluscs: a role of epidermal ionocytes in acid-base regulation during embryogenesis.

These findings add significant knowledge to the mechanistic understanding of hypercapnia tolerance in marine organisms, as it demonstrates that marine taxa, which were identified as powerful acid-base regulators during hypercapnic challenges, already exhibit strong acid- base regulatory abilities during embryogenesis.

Prophenoloxidase system, lysozyme and protease inhibitor distribution in the common cuttlefish Sepia officinalis.



Ultrastructural and cytochemical investigations on the renal appendages and their concrements in dibranchiate cephalopods (Mollusea, Cephalopoda)

SummaryThe renal appendages of the octopods Octopus vulgaris Lam., Eledone moschata Lam. and of the decapods of the order Sepioidea Sepia officinalis L., and of sea water suggest osmotic filtration

The localization of enzyme activities in the pancreatic appendages of Sepia officinalis L. (Cephalopoda)

Enzyme activities of the pancreatic appendages of the ductus hepatopancreas in Sepia officinalis L. have been demonstrated by light and electron microscopical methods, and the presumed function of the two pancreatic epithelia is discussed.

Fine structure of the respiratory lamellae of teleostean gills

  • J. Newstead
  • Biology
    Zeitschrift für Zellforschung und Mikroskopische Anatomie
  • 2004
SummaryThe blood-water pathway in respiratory lamellae of teleostean gills consists of an epithelial layer one or two cells thick, a basal lamina and a thin layer of cytoplasm which lines the blood


A morphological basis for osmoregulation in the teleosts was studied by comparing the fine structure of chloride cells found in epithelia of the gills of three species of fish: Fundulus heteroclitus, F. similis and F. chrysotus which are usually restricted to salt water and fresh water environments, respectively.

Elektronenmikroskopische und histochemische Untersuchungen zur Funktion des Kiemenherzanhanges (Pericardialdrüse) von Sepia officinalis

SummaryOur electron microscopic studies of the appendages of the branchial heart and veins of Sepia officinalis suggest an excretory function for these organs. The pericardial gland appears to be a

The excretion of NH4+ and its ouabain sensitivity in aquatic annelids and molluscs

Results indicate that ammonia crosses the excretory epithelium in the ionized form, and that the process is linked to the activity of the Na+ + K+ ATPases.

[Histochemical evidence of aminotransferases. IV. Histochemical and electrophoretical investigation of aminotransferases in rat organs (author's transl)].

The studied aminotransferases possessed isoenzyme fractions with characteristic number, electrophoretic mobility and intensity in the different organs of the rat and the results were discussed in relation with substrate specifity of the aminOTransferase.


Localization of the formazan to autonomic ganglia and fibers, chief cells of the gastric fundus, mucosal epithelium of the duodenum and certain specific zones of the renal tubules by means of the tryptamine-tetrazolium technique was confirmed as indicating monoamine oxidase activity by identical localization using the hydrazone precipitation technique of Koelle and Valk.

Further Studies on Ionic Regulation in Marine Invertebrates

In sixteen crustaceans an inverse relationship exists between the degree of activity and the magnesium content of the blood: the more active ones have low values of magnesium, and in the grapsoid crab Pachygrapsus each ion in the plasma is maintained below its equilibrium value.


The synthesis of a substrate, 6-bromo-2-naphthyl β-d-glucopyruronoside, is described, and the procedure for the histochemical demonstrations of β-d-glucuronidase activity and experiments to determine