Electron microscopic mappings of myosin head with site-directed antibodies.

@article{Sutoh1989ElectronMM,
  title={Electron microscopic mappings of myosin head with site-directed antibodies.},
  author={Kazuo Sutoh and Michiyo Tokunaga and Tokumitsu Wakabayashi},
  journal={Journal of molecular biology},
  year={1989},
  volume={206 2},
  pages={
          357-63
        }
}
Site-directed antibodies were raised against three synthetic peptides whose sequences correspond to a region around the reactive lysine residue and two protease-sensitive regions of subfragment 1 (S1) of skeletal muscle myosin (one at the junction of the 23,000 Mr and 50,000 Mr segments, the J1 junction; and the other at the junction of the 50,000 Mr and 20,000 Mr segments of the heavy chain, the J2 junction). The antisera cross-reacted with intact myosin with titres of 5 x 10(4) (anti-J1… CONTINUE READING

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