Electron microscopic-immunocytochemical localization of adrenocorticotropin and melanocyte stimulating hormone in the pars intermedia cells of rats and mice

@article{Naik2004ElectronML,
  title={Electron microscopic-immunocytochemical localization of adrenocorticotropin and melanocyte stimulating hormone in the pars intermedia cells of rats and mice},
  author={D. Naik},
  journal={Zeitschrift f{\"u}r Zellforschung und Mikroskopische Anatomie},
  year={2004},
  volume={142},
  pages={305-328}
}
  • D. Naik
  • Published 2004
  • Biology, Medicine
  • Zeitschrift für Zellforschung und Mikroskopische Anatomie
SummaryElectron microscopic localization of adrenocorticotropin (ACTH) and melanocyte stimulating hormone (MSH) in light, dark and ACTH cells in the pars intermedia (PI) of rats and mice is attempted by using antisera to βp 1–24, βp 17–39 ACTH and βb MSH with the immunoglobulin-peroxidase bridge technique. All of the PI parenchymatous cells (light, dark and ACTH cells), except the marginal cuboidal and the ependymal like cells, in rats and mice show very good localization of ACTH and MSH… Expand
Immunohistochemical localization of adrenocorticotropin and melanocyte stimulating hormone in pars intermedia of rat hypophysis
  • D. Naik
  • Biology, Medicine
  • Zeitschrift für Zellforschung und Mikroskopische Anatomie
  • 2004
TLDR
It seems to me that the major amount of corticotropin is supplied by the ACTH cells of the PD, PI and PT, and less by the light glandular and dark Cells of the PI. Expand
The utility of antisera to different synthetic adrenocorticotrophins (ACTH) and melanotrophins (MSH) for immunocytochemical staining of the dog pituitary gland
TLDR
It is concluded that ACTH and MSHβ were present and most probably produced by the corticomelanotrophs of the pars distalis and pars tuberalis and by immunochemical double staining. Expand
Immuno-electron microscopic localization of luteinizing hormone-releasing hormone in the arcuate nuclei and median eminence of the rat
  • D. Naik
  • Biology, Medicine
  • Cell and Tissue Research
  • 2004
TLDR
It was concluded that the LH-RH is packaged or condensed into granules in the Golgi cisternae. Expand
Immunoreactive LH-RH neurons in the hypothalamus identified by light and fluorescent microscopy
  • D. Naik
  • Biology, Medicine
  • Cell and Tissue Research
  • 2004
SummaryThe sites of luteinizing hormone-releasing hormone (LH-RH) neurons and their axonal pathways in the hypothalami of rats and mice were studied by the immuno-globulin-peroxidase bridge techniqueExpand
Pituitary Polypeptide-Secreting Cells: Immunocytochemical Identification of ACTH, MSH, and Peptides Related to LPH
Until recently, the anterior lobe of the hypophysis was thought to be responsible for corticotropin (ACTH) elaboration, while melanotropin(MSH) secretion was attributed to the intermediate lobe.Expand
Ontogenesis of the α-MSH, β-MSH and ACTH cells in the foetal hypophysis of the rat. Correlation with the growth of the adrenals and adrenocortical activity
TLDR
The data suggest that hypophyseal corticostimulating activity is very high between days 18 and 19 and decreases between days 19 and 21, and that Adrenal and plasma corticosterone concentrations reach a peak on day 19 of pregnancy. Expand
Electron microscopical study on the development of the nerve supply of the pituitary pars intermedia of the mouse
  • R. Jarskär
  • Biology, Medicine
  • Cell and Tissue Research
  • 2004
TLDR
The development of the nerve supply of the pituitary pars intermedia of C3H mice was studied by electron microscopy, indicating that the primary innervation is exerted by adrenergic neurons. Expand
Characterization by different techniques of adrenocorticotropin and gonadotropin producing cells in lerot pituitary (Eliomys quercinus)
TLDR
The superimposition technique allows an excellent description of cell ultrastructure of individually identified cells of each type and was able to desdribe the corticotropin secreting cells as lucent cells with electron dense granules ranging in size from 2500 to 3500 Å. Expand
Development and cytodifferentiation of the rabbit pars intermedia
TLDR
Four groups of parenchyma cells are recognisable: Pi-glandular cells, interstitial cells, ACT-type cells and a heterogeneous group of dark cells, which has been traced from 14 days PC to 32 days PC. Expand
Three-dimensional ultrastructural analyses of anterior pituitary gland expose spatial relationships between endocrine cell secretory granule localization and capillary distribution
TLDR
Three-dimensional cellular architectures of the anterior pituitary tissue at ultrastructural resolution suggest that the cellular interactions between the endocrine and endothelial cells promote an uneven cytoplasmic distribution of the secretory granules. Expand
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References

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Immunohistochemical localization of adrenocorticotropin and melanocyte stimulating hormone in pars intermedia of rat hypophysis
  • D. Naik
  • Biology, Medicine
  • Zeitschrift für Zellforschung und Mikroskopische Anatomie
  • 2004
TLDR
It seems to me that the major amount of corticotropin is supplied by the ACTH cells of the PD, PI and PT, and less by the light glandular and dark Cells of the PI. Expand
Adrenocorticotropin production by the intermediate lobe of the rat pituitary
TLDR
There were distinct immunohistochemical differences between the pars intermedia and anterior lobe ACTH cells, which suggests that ACTH may not be packaged or condensed in the Golgi region of the latter. Expand
Functional morphology of the pars intermedia of the rat hypophysis as revealed with the electron microscope
  • Yasuo Kobayashi
  • Biology, Medicine
  • Zeitschrift für Zellforschung und Mikroskopische Anatomie
  • 2004
TLDR
Observations of the hypophysis at various intervals following formalin induced stress or adrenalectomy indicate the existence of a functional relationship between the posterior lobe, the pars intermedia, and the adrenal cortex. Expand
Electron microscopic studies on the pars intermedia in normal and in mice with hereditary nephrogenic diabetes insipidus
  • D. Naik
  • Biology, Medicine
  • Zeitschrift für Zellforschung und Mikroskopische Anatomie
  • 2004
TLDR
It is concluded that the dense core granules in PI may be synthesizing ACTH or ACTH-like substance and may further mature and give rise to MSH in the form of electron lucent vesicles. Expand
CLASSIFICATIONS OF ANTERIOR PITUITARY CELL TYPES WITH IMMUNOENZYME HISTOCHEMISTRY
  • P. Nakane
  • Biology, Medicine
  • The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • 1970
TLDR
Peroxidase-labeled antibody method was used to localize the six hormones of the anterior pituitary gland of male rats both at the light and electron microscopic levels, and gonadotropic cells may be identified but their hormone content cannot be determined. Expand
Electron microscopy of the pars intermedia of the pituitary gland in the rat.
TLDR
A system of extracellular channels between the cells of the pars intermedia in continuity with dilatations of the Extracellular space, is described, and the findings are discussed in relation to the possible secretory function of the Pars inter media in mammals. Expand
The cellular origins of corticotropin and melanotropin as revealed by immunochemical staining.
TLDR
Observations suggest that the pars distalis is primarily responsible for supplying the corticotropin required to meet the changing requirements of the body. Expand
Functional morphology of the pars intermedia of the rat hypophysis as revealed with the electron microscope. II. Correlation of the pars intermedia with the hypophyseo-adrenal axis.
  • Y. Kobayashi
  • Chemistry, Medicine
  • Zeitschrift fur Zellforschung und mikroskopische Anatomie
  • 1965
TLDR
Results apparently indicated that the release of granule content of the light cells of the Pars intermedia was inhibited by dexamethasone which had been known to prevent a release of ACTH from the adenohypophysis, and that the pars intermedia responded to stressful stimuli such as adrenalectomy and laparotomy, showing morphological alterations of the density of secretory granules. Expand
Electron microscope studies on the fine structures of the pars nervosa and pars intermedia, and their morphological interrelation in the normal rat hypophysis.
TLDR
Pars nervosa and pars intermedia in the hypophyses of the adult male albino rats fed in normal condition were observed with the electron microscope, and small numbers of dense granules were observed at the Golgi apparatus of the light cells of Pars intermedia, suggesting secretory activities which have not been completely clarified at the present state of investigation. Expand
Electron microscopic studies on the pars intermedia of the ferret
TLDR
The pattern of innervation in the ferret is comparable to that previously observed in the cat, rather than that seen in rodents or monkeys, and the implications of this finding are discussed. Expand
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