Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes

@article{Pan1985ElectronME,
  title={Electron microscopic evidence for externalization of the transferrin receptor in vesicular form in sheep reticulocytes},
  author={B T Pan and Kelly Teng and Chuanchu Wu and Myriam Adam and Rose M. Johnstone},
  journal={The Journal of Cell Biology},
  year={1985},
  volume={101},
  pages={942 - 948}
}
Using ferritin-labeled protein A and colloidal gold-labeled anti-rabbit IgG, the fate of the sheep transferrin receptor has been followed microscopically during reticulocyte maturation in vitro. After a few minutes of incubation at 37 degrees C, the receptor is found on the cell surface or in simple vesicles of 100-200 nm, in which the receptor appears to line the limiting membrane of the vesicles. With time (60 min or longer), large multivesicular elements (MVEs) appear whose diameter may… CONTINUE READING