Electron microscopic demonstration of nick end-labeled DNA fragments during capsaicin-induced apoptosis of trigeminal primary neurons in neonatal rats.

@article{Sugimoto1999ElectronMD,
  title={Electron microscopic demonstration of nick end-labeled DNA fragments during capsaicin-induced apoptosis of trigeminal primary neurons in neonatal rats.},
  author={Tomosada Sugimoto and Aya Takeyama and Cheng Xiao and Teruko Takano-Yamamoto and Hiroyuki Ichikawa},
  journal={Brain research},
  year={1999},
  volume={818 1},
  pages={147-52}
}
DNA fragmentation was induced in the trigeminal ganglion of newborn rats by subcutaneous capsaicin injection (50 mg/kg). Twenty-four hours later, numerous roundish profiles were intensely labeled by both a DNA polymerase I-mediated nick translation method and a terminal transferase-mediated tailing method. Direct electron microscopic examination of labeled profiles indicated that the labeled profiles were neurons at earlier stages of apoptosis. DNA fragmentation signal was first detected in the… CONTINUE READING