Electron microscopic and molecular characterization of turnip vein-clearing virus

@article{Lartey1994ElectronMA,
  title={Electron microscopic and molecular characterization of turnip vein-clearing virus},
  author={Robert T. Lartey and Leslie C. Lane and U. Melcher},
  journal={Archives of Virology},
  year={1994},
  volume={138},
  pages={287-298}
}
We recently isolated turnip vein-clearing virus (TVCV), a tobamovirus which causes vein clearing inBrassica rapa (turnip) and a mosaic inNicotiana tabacum (tobacco). We present an electron microscopic and molecular characterization of TVCV. Viral particles from lower epidermis peel contained rod-shaped viral particles, typical of tobamoviruses. Viral RNA extracted from infected turnip leaves was used as template for cDNA synthesis prior to cloning in a plasmid vector. Inserts of selected cDNA… CONTINUE READING

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The nucleotide and predicted amino acid sequences were 56 to 59% identical to those of most other sequenced tobamoviruses .
The nucleotide and predicted amino acid sequences were 56 to 59% identical to those of most other sequenced tobamoviruses .
The least related sequence , that of cucumber green mottle mosaic virus , was more related to the TVCV lineage than it was to those of the other sequenced tobamoviruses .
The least related sequence , that of cucumber green mottle mosaic virus , was more related to the TVCV lineage than it was to those of the other sequenced tobamoviruses .
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