Electron microscopic analysis of RNA transcription in preimplantation rabbit embryos

@article{Cotton1980ElectronMA,
  title={Electron microscopic analysis of RNA transcription in preimplantation rabbit embryos},
  author={Robin W. Cotton and Cole Manes and Barbara A. Hamkalo},
  journal={Chromosoma},
  year={1980},
  volume={79},
  pages={169-178}
}
We have examined directly RNA synthesis in several stages of preimplantation rabbit embryos by electron microscopic analysis of chromatin spreads. DNA/chromatin packing ratios were determined for non-transcribing regions (2.0) and for regions active in RNA synthesis (1.1). Significant amounts of RNA transcription were observed in all embryonic stages examined. Lengths of transcribed regions were calculated and range from 1.1 to 10.9 Kb of B-form DNA. The frequency of chromatin-associated… CONTINUE READING