• DOI:10.1007/BF00331801
• Corpus ID: 32146677

Electron microscope observations of the organisation of chromatin fibers in isolated nuclei of rat liver

@article{Heumann2004ElectronMO,
  title={Electron microscope observations of the organisation of chromatin fibers in isolated nuclei of rat liver},
  author={H G Heumann},
  journal={Chromosoma},
  year={2004},
  volume={47},
  pages={133-146}
}

• H. Heumann
• Published 2004
• Biology
• Chromosoma

In this study a technique is used which allows observation of chromatin fibers in sections of isolated liver nuclei of the rat. The fibers, which exhibit a rather uniform diameter (diameter = 125 ± 20 Å) seem to be constructed of coiled or folded sub-filaments. — After application of a preferential ribonucleoproteinstain (EDTA-method) only parts of the fibers retain their stain whereas others are completely bleached. Finer filaments (diameter = 45 Å) seem to be continuous with the stained parts of the chromatin fibers. The RNP-stain is lost when the nuclei have been treated with RNase. From these observations it is concluded that RNA-rich and RNA-free segments exist on chromatin fibers which may be related to RNA transcription processes. — Granules and thin filaments are the only structures stained with the EDTA-method in the nucleolus.