Electron diffraction of frozen, hydrated protein crystals.

@article{Taylor1974ElectronDO,
  title={Electron diffraction of frozen, hydrated protein crystals.},
  author={Kenneth A. Taylor and Robert M. Glaeser},
  journal={Science},
  year={1974},
  volume={186 4168},
  pages={1036-7}
}
High-resolution electron diffraction patterns have been obtained from frozen, hydrated catalase crystals to demonstrate the feasibility of using a frozen specimen hydration technique. The use of frozen specimens to maintain the hydration of complex biological structures has certain advantages over previously developed liquid hydration techniques. 

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