Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation.

@article{Salje2009ElectronCO,
  title={Electron cryomicroscopy of E. coli reveals filament bundles involved in plasmid DNA segregation.},
  author={Jeanne Salje and Beno{\^i}t Zuber and Jan L{\"o}we},
  journal={Science},
  year={2009},
  volume={323 5913},
  pages={509-12}
}
Bipolar elongation of filaments of the bacterial actin homolog ParM drives movement of newly replicated plasmid DNA to opposite poles of a bacterial cell. We used a combination of vitreous sectioning and electron cryotomography to study this DNA partitioning system directly in native, frozen cells. The diffraction patterns from overexpressed ParM bundles in electron cryotomographic reconstructions were used to unambiguously identify ParM filaments in Escherichia coli cells. Using a low-copy… CONTINUE READING

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