Electron-capture GLC determination of bromhexine in human plasma.

  title={Electron-capture GLC determination of bromhexine in human plasma.},
  author={A P De leenheer and L M Vandecasteele-Thienpont},
  journal={Journal of pharmaceutical sciences},
  volume={69 1},
A specific and sensitive GLC analysis of bromhexine in human plasma is described. After addition of the internal standard and an aqueous triethanolamine solution, bromhexine is extracted at alkaline pH into n-hexane, transferred to an acidic aqueous solution, and back-extracted into n-hexane after alkalinization. Both compounds are derivatized with trifluoroacetic anhydride and quantified by GLC using , 63Ni-electron-capture detector. The method has a sensitivity of approximately 1.0 ng/ml of… CONTINUE READING