Electron and scanning force microscopy studies of alterations in supercoiled DNA tertiary structure.

@article{Cherny2001ElectronAS,
  title={Electron and scanning force microscopy studies of alterations in supercoiled DNA tertiary structure.},
  author={Dmitry I. Cherny and Thomas M. Jovin},
  journal={Journal of molecular biology},
  year={2001},
  volume={313 2},
  pages={295-307}
}
The configuration of supercoiled DNA (scDNA) was investigated by electron microscopy and scanning force microscopy. Changes in configuration were induced by varying monovalent/divalent salt concentrations and manifested by variation in the number of nodes (crossings of double helical segments). A decrease in the concentration of monovalent cations from 50 mM to approximately 1 mM resulted in a significant change of apparent configuration of negatively supercoiled DNA from a plectonemic form… CONTINUE READING