Electron Microscopy and Molecular Characterization of Phytoplasmas Associated with Strawflower Yellows in the Czech Republic

@article{Frnov2004ElectronMA,
  title={Electron Microscopy and Molecular Characterization of Phytoplasmas Associated with Strawflower Yellows in the Czech Republic},
  author={Jana Fr{\'a}nov{\'a} and Jaroslava Přibylov{\'a} and Marie {\vS}imkov{\'a} and Milan Navr{\'a}til and Pavla V{\'a}lov{\'a}},
  journal={European Journal of Plant Pathology},
  year={2004},
  volume={109},
  pages={883-887}
}
Strawflower (Helichrysum bracteatum) with symptoms resembling those associated to phytoplasma infection were observed in several areas in the Czech Republic during the period 1994–2001. Plants with leaf bronzing, reddening and necrosis, proliferation of secondary shoots, flower abnormalities and dwarfing died in advanced stages of the disease. The disease incidence ranged from 2% to 70% and caused significant loss to the flower and seed production. Transmission electron microscopy showed… 
Phytoplasma associated with witches’-broom disease of Ulmus minorMill. in the Czech Republic: Electron microscopy and molecular characterization
TLDR
Visual inspections of elm trees in south Moravia in 1997–2007 revealed a rare occurrence of plants with smaller and cowl-forming leaves on some twigs, i.e. a feature resembling witches’-broom disease observed on the end of twig, which is the northernmost confirmed occurrence of phytoplasma onElm trees within Europe.
First report of ‘Candidatus Phytoplasma aurantifolia’(16SrII-A) associated with strawflower phyllody
Four strawflower plant samples showing phyllody disease symptoms were collected from the experimental plot at the College of Horticulture, Bangalore, Karnataka (India). The phytoplasma infection in
Visualization of phytoplasmas using electron microscopy.
TLDR
Now that the full sequencing of four phytoplasma genomes has been achieved, electron microscopy can play an important role in identifying and understanding specific gene functions.

References

SHOWING 1-10 OF 15 REFERENCES
Association of aster yellows subgroup 16SrI-B phytoplasmas with a disease of Rehmannia glutinosa var. purpurea
TLDR
The phytoplasma in the subgroup 16SrI-B is confirmed in naturally infected samples of R. purpurea and in symptomatic periwinkle after dodder transmission of the agent.
Aster Yellows Phytoplasma Identified in Scentless Chamomile by Microscopical Examinations and Molecular Characterization
TLDR
Application of 4′6-diamidino-2-phenylindole- 2HCl (DAPI) staining techniques confirmed the presence of the phytoplasma in these tissues examined by electron microscopy and supported by polymerase chain reaction (PCR) assays, using two primer pairs derived from phy toplasma rDNA sequences.
Detection and differentiation of phytoplasmas in Australia: an update
TLDR
Seven new restriction fragment patterns have been detected and sequence analysis of the 16S/23S spacer region revealed that 3 of these phytoplasmas are related to the faba bean phyllody (FBP) group, members of phy toplasmas groups that have not been reported before in Australia.
Detection of the apple proliferation and pear decline phytoplasmas by PCR amplification of ribosomal and nonribosomal DNA.
TLDR
Results show that it is not possible to detect all strains of the AP and PD phytoplasmas, respectively, with pathogen-specific primers.
Genetic Interrelatedness among Clover Proliferation Mycoplasmalike Organisms (MLOs) and Other MLOs Investigated by Nucleic Acid Hybridization and Restriction Fragment Length Polymorphism Analyses
TLDR
Similarity coefficients derived from restriction fragment length polymorphism analyses revealed that PWB and CP MLOs are closely related strains and thus provided direct evidence of their relatedness in contrast to reliance solely on biological characterization.
Ornamental flowering plants naturally infected with curly-top and aster-yellows viruses
TLDR
The host range of virus diseases as determined by experimental infection often does not coincide with the food and breeding plants preferred by the insect vector of the disease under natural conditions.
Sensitive detection and identification of mycoplasma-like organisms in plants by polymerase chain reactions.
Revised Classification Scheme of Phytoplasmas based on RFLP Analyses of 16S rRNA and Ribosomal Protein Gene Sequences
TLDR
The phytoplasma 16Sr groups were consistent with the phylogenetic groups (subclades) defined by phylogenetic analysis of near-full-length 16S rRNA gene sequences, indicating that the RFLP-based groups are phylogenetically valid.
Current status of molecular classification of the phytoplasmas.
TLDR
A total of 75 phy toplasmas were distinguishable among the molecularly characterized phytopathogenic mollicutes, and these were classified into 20 major phylogenetic groups or subclades.
Association of aster yellows subgroup 16SrI-B phytoplasma with a disease of Rehmannia glutinosa var
  • purpurea. Plant Pathology
  • 2001
...
...