Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division

@article{Chapman1959ElectronMO,
  title={Electron Microscope Observations on the Behavior of the Bacterial Cytoplasmic Membrane During Cellular Division},
  author={George B. Chapman},
  journal={The Journal of Biophysical and Biochemical Cytology},
  year={1959},
  volume={6},
  pages={221 - 224}
}
Bacterial cells were fixed in OsO(4), washed, dehydrated, and embedded in a methacrylate mixture. Ultrathin sections were cut on a Porter-Blum ultramicrotome and were examined in an RCA electron microscope, type EMU-2D. The sections revealed that the cytoplasmic membrane undergoes a centripetal growth to form a membrane septum. This septum is formed as a double structure. Constriction of the daughter cells and deposition of cell wall material lead to the separation of the daughter cells. The… CONTINUE READING
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