Corpus ID: 10788830

Electron Microscope Characterization of Carbohydrate Residues on the Body Wall of Xiphinema index '

@inproceedings{Spiegfl2001ElectronMC,
  title={Electron Microscope Characterization of Carbohydrate Residues on the Body Wall of Xiphinema index '},
  author={Y. Spiegfl},
  year={2001}
}
T h e location of carbohydrate moieties on the nuter cuticle of Xiphinema index was examined by electron mic rocopy using several different reagents: a) The periodic acidthiosemicarbazide-silver proteiuate reaction was used as a general stain for carbohydrates. In sectioned material it stained the canal system and deeper layers of the cuticle as well as the outer surface, b) Cationized ferritin at pH 2.5, which identifies carboxyl and sulfate groups, was used to identify sialic acid residues… Expand
2 Citations
Tissue treatment for whole mount internal lectin staining in the nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus
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Acetone-only fixation was found to give strong and reproducible staining, which could be prevented either by periodate treatment of the organisms or by specific inhibitory sugars of the lectins under investigation. Expand
Review of the ultrastructure of the nematode body cuticle and its phylogenetic interpretation
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The phylogenetic interpretation of the nematode cuticle ultrastructure is reviewed within the framework of recent DNA‐sequence data and the cuticle appears to be unreliable regarding resolution of deep‐level relationships in the Nematoda. Expand

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