Corpus ID: 10788830

Electron Microscope Characterization of Carbohydrate Residues on the Body Wall of Xiphinema index '

  title={Electron Microscope Characterization of Carbohydrate Residues on the Body Wall of Xiphinema index '},
  author={Y. Spiegfl},
T h e location of carbohydrate moieties on the nuter cuticle of Xiphinema index was examined by electron mic rocopy using several different reagents: a) The periodic acidthiosemicarbazide-silver proteiuate reaction was used as a general stain for carbohydrates. In sectioned material it stained the canal system and deeper layers of the cuticle as well as the outer surface, b) Cationized ferritin at pH 2.5, which identifies carboxyl and sulfate groups, was used to identify sialic acid residues… Expand
2 Citations
Tissue treatment for whole mount internal lectin staining in the nematodes Caenorhabditis elegans, Panagrolaimus superbus and Acrobeloides maximus
Acetone-only fixation was found to give strong and reproducible staining, which could be prevented either by periodate treatment of the organisms or by specific inhibitory sugars of the lectins under investigation. Expand
Review of the ultrastructure of the nematode body cuticle and its phylogenetic interpretation
The phylogenetic interpretation of the nematode cuticle ultrastructure is reviewed within the framework of recent DNA‐sequence data and the cuticle appears to be unreliable regarding resolution of deep‐level relationships in the Nematoda. Expand


Characterization of sialyl and galactosyl residues on the body wall of different plant parasitic nematodes.
The existence and nature of sugar residues on the cuticle surface of nematodes could contribute to an understanding of the specific recognition by phytophagous nem atodes of their host, and perhaps also of the virus transmission mechanism in those nematode which serve as vectors. Expand
Localization of sialyl residues on cell surfaces by affinity cytochemistry.
The frequency of attached ferritin—avidin conjugates to biotinylated erythroid cells in rabbit bone marrow was found to vary according to the degree of maturation in the erythrocyte line, corresponding to the previously described variations in colloidal iron binding. Expand
Caenorhabditis briggsae: aging and the structural turnover of the outer cuticle surface and the intestine.
It is suggested that there is no turnover of acid mucopolysaccharides after the final molt of C. briggsae and this surface coat appeared to shrink with age. Expand
Ferritin hydrazide, a novel covalent electron dense reagent for the ultrastructural localization of glycoconjugates.
This study represents the first example of covalent labeling of cell surfaces with an electron dense material and is much simpler then the three-step affinity cytochemical techniques based on avidin-biotin interaction. Expand
Partial Characterization of the Cuticle Surface of Meloidogyne javanica Females.
Evidence is presented that the electronegative charge is not borne on neuraminic acid, and a surface coat, or glycocalyx, external to the outer cuticle membrane was demonstrated. Expand
Action of Vibrio cholerae neuraminidase (sialidase) upon the surface of intact cells and their isolated sialolipid components.
It is suggested that the sialolipid components in the intact cell surface are relatively unavailable to the extracellular action of VC-neuraminidase, and release of sialic acid from intact cells by VC neuraminid enzyme appears to be from surface sialoglycoproteins. Expand
Differential characterization of carboxyl and sulfate groups in thin sections for electron microscopy
  • Biol. Celhd. 36:281-288
  • 1979
Staining methods for sectioned material
Membrane Glycoproteins: A Review of Structure and Function