Electron Microscope Analysis of Young and Old Red Blood Cells Stained with Colloidal Iron for Surface Charge Evaluation

@article{Marikovsky1969ElectronMA,
  title={Electron Microscope Analysis of Young and Old Red Blood Cells Stained with Colloidal Iron for Surface Charge Evaluation},
  author={Yehuda Marikovsky and David Danon},
  journal={The Journal of Cell Biology},
  year={1969},
  volume={43},
  pages={1 - 7}
}
Human and rabbit red blood cells, separated into "young" and "old" age groups by differential flotation on phthalate esters, were fixed with glutaraldehyde and labeled with colloidal ferric oxide. Electron micrographs of thin sections of young cells showed a uniform and dense depostion of positive iron particles. Old cells showed particles deposited irregularly, leaving unlabeled gaps on the membrane surface. Red cells incubated with 10 units/ml receptor-destroying enzyme (RDE) demonstrate a… CONTINUE READING

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