Electromagnetic re-warming of cryopreserved tissues: effect of choice of cryoprotectant and sample shape on uniformity of heating.

@article{Robinson2002ElectromagneticRO,
  title={Electromagnetic re-warming of cryopreserved tissues: effect of choice of cryoprotectant and sample shape on uniformity of heating.},
  author={Martin P. Robinson and Monica C Wusteman and Lihong Wang and David E. Pegg},
  journal={Physics in medicine and biology},
  year={2002},
  volume={47 13},
  pages={2311-25}
}
A method that has been proposed for the cryopreservation of tissues and organs is to add a cryoprotective agent (CPA) in sufficient concentration to allow vitrification, and to use rapid electromagnetic heating to prevent the formation of ice crystals during the re-warming. We have compared the physical and biological properties of four CPAs, measuring the speed and uniformity of heating in a 36 mm sphere placed in a 434 MHz applicator, and the toxicity to ECV304 endothelial cells. Ethanediol… CONTINUE READING