Electrogenic H+ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme.

@article{Perlin1984ElectrogenicHT,
  title={Electrogenic H+ translocation by the plasma membrane ATPase of Neurospora. Studies on plasma membrane vesicles and reconstituted enzyme.},
  author={David Scott Perlin and Kunihiro Kasamo and Robert J. Brooker and Carolyn W. Slayman},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 12},
  pages={7884-92}
}
Fluorescent probes have been used to measure electrogenic proton pumping by the plasma membrane ATPase of Neurospora. In isolated plasma membrane vesicles, greater than 85% of which are inverted, ATP hydrolysis is accompanied by the formation of an inside acid pH gradient (delta pH) which can be detected by acridine orange fluorescence quenching and an inside positive membrane potential (delta psi) which can be detected by oxonol V fluorescence quenching. Maximal values of delta pH were… CONTINUE READING
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