Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells.

@article{Tatham1996ElectrofusionPF,
  title={Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells.},
  author={Brendan G Tatham and K J Giliam and Alan O. Trounson},
  journal={Molecular reproduction and development},
  year={1996},
  volume={43 3},
  pages={306-12}
}
Electrofusion is a valuable technique for the nuclear transfer procedure. An enucleated oocyte is electrofused with a blastomere to create a nuclear transfer embryo. The present study constructed isofusion contours after the electrofusion of identical coupled cells that characterized all the bovine embryonic cell types used in nuclear transfer. The intersection of isofusion contours for enucleated oocytes and blastomeres provided the parameters for electrofusion during nuclear transfer… CONTINUE READING

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