Electrofusion-induced intracellular Ca2+ flux and its effect on murine oocyte activation.

@article{Rickords1992ElectrofusioninducedIC,
  title={Electrofusion-induced intracellular Ca2+ flux and its effect on murine oocyte activation.},
  author={Lee F. Rickords and Kenneth L. White},
  journal={Molecular reproduction and development},
  year={1992},
  volume={31 2},
  pages={152-9}
}
These experiments were designed to monitor influx of extracellular Ca2+ into the murine ooplasm following a 1.56 kV.cm-1 direct current (DC) electrofusion pulse and subsequently to determine its effect on rate of activation. Pulse media consisted of non-electrolyte (0.3 M mannitol) and electrolyte (phosphate-buffered saline; PBS) media each containing 0.0, 0.05, or 0.9 mM Ca2+ (groups T1-T3 and T4-T6, respectively). Cumulus-free oocytes were incubated in 100 microliters drops of PBS containing… CONTINUE READING
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