Electrofusion between heterogeneous-sized mammalian cells in a pellet: potential applications in drug delivery and hybridoma formation.

@article{Li1996ElectrofusionBH,
  title={Electrofusion between heterogeneous-sized mammalian cells in a pellet: potential applications in drug delivery and hybridoma formation.},
  author={Li H. Li and Mary L. Hensen and Yan Long Zhao and Sek Wen Hui},
  journal={Biophysical journal},
  year={1996},
  volume={71 1},
  pages={
          479-86
        }
}
High-efficiency electrofusion between cells of different sizes was achieved by application of fusing electric pulses to cells in centrifuged pellets. Larger target cells (Chinese hamster ovary or L1210 cells) were stacked among smaller human erythrocytes or erythrocyte ghosts by sequential centrifugation at 700 g to form five-tier pellets in a specially designed centrifugation-electrofusion chamber. The membranes of erythrocytes and ghost were labeled with fluorescent membrane dye (1,1… CONTINUE READING

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