Electrochemical quantification of serotonin in the live embryonic zebrafish intestine.

@article{Njagi2010ElectrochemicalQO,
  title={Electrochemical quantification of serotonin in the live embryonic zebrafish intestine.},
  author={John I. Njagi and Michael Ball and Marc Best and Kenneth N Wallace and Silvana Andreescu},
  journal={Analytical chemistry},
  year={2010},
  volume={82 5},
  pages={1822-30}
}
We monitored real-time in vivo levels of serotonin release in the digestive system of intact zebrafish embryos during early development (5 days postfertilization, dpf) using differential pulse voltammetry with implanted carbon fiber microelectrodes modified with carbon nanotubes dispersed in nafion. A detection limit of 1 nM, a linear range between 5 and 200 nM, and a sensitivity of 83.65 nA x microM(-1) were recorded. The microelectrodes were implanted at various locations in the intestine of… CONTINUE READING