Electrochemical oxidation of cholesterol catalyzed by cholesterol oxidase with use of an artificial electron mediator.

@article{Nakaminami1997ElectrochemicalOO,
  title={Electrochemical oxidation of cholesterol catalyzed by cholesterol oxidase with use of an artificial electron mediator.},
  author={Takeshi Nakaminami and Susumu Kuwabata and Hiroshi Yoneyama},
  journal={Analytical chemistry},
  year={1997},
  volume={69 13},
  pages={2367-72}
}
Several redox dyes of phenazine and phenothiazine derivatives including 1-methoxy-5-methylphenazinium methosulfate (MPMS), thionin, azure A, and azure C work well as electron acceptors for reduced cholesterol oxidase (EC 1.1.3.6) (ChOx). Kinetic parameters and equilibrium constants of the enzymatic oxidation of cholesterol obtained suggest that the… CONTINUE READING