Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum.

@article{Jarocka2014ElectrochemicalIF,
  title={Electrochemical immunosensor for detection of antibodies against influenza A virus H5N1 in hen serum.},
  author={Urszula Jarocka and R{\'o}za Sawicka and Anna G{\'o}ra-Sochacka and Agnieszka Sirko and Włodzimierz Zag{\'o}rski-Ostoja and Jerzy Radecki and Hanna Radecka},
  journal={Biosensors & bioelectronics},
  year={2014},
  volume={55},
  pages={
          301-6
        }
}
This paper describes the development of an immunosensor for detection of anti-hemagglutinin antibodies. Its preparation consists of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with EDC/NHS coupling reaction, (iii) covering with anti-His IgG monoclonal antibody, (iv) immobilization of the recombinant His-tagged hemagglutinin (His6-H5 HA), (v) filling free space with BSA. The interactions between two variants of… CONTINUE READING

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References

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