Electrochemical Studies of Polynucleotide Binding and Oxidation by Metal Complexes: Effects of Scan Rate, Concentration, and Sequence

@inproceedings{Sistare1999ElectrochemicalSO,
  title={Electrochemical Studies of Polynucleotide Binding and Oxidation by Metal Complexes: Effects of Scan Rate, Concentration, and Sequence},
  author={Mark Foster Sistare and Rebecca C. Holmberg and H. Holden Thorp},
  year={1999}
}
The electrocatalytic oxidation of guanine in DNA and oligonucleotides by Ru(bpy)33+/2+ was investigated using cyclic voltammetry (CV) and chronoamperometry (CA) (bpy = 2,2‘-bipyridine). Oxidation of Ru(bpy)32+ to the Ru(III) form at tin-doped indium oxide (ITO) electrodes in the presence of DNA produces catalytic current due to the oxidation of guanine by Ru(III). CA traces of Ru(bpy)32+ with calf thymus DNA at high salt concentration (50 mM sodium phosphate + 700 mM NaCl) give a single kinetic… CONTINUE READING