Electro-optic response of second-harmonic generation membrane potential sensors.

  title={Electro-optic response of second-harmonic generation membrane potential sensors.},
  author={Laurent Moreaux and Thomas Pons and V Dambrin and Mireille Blanchard-Desce and Jerome Mertz},
  journal={Optics letters},
  volume={28 8},
We quantify and ascertain the nature of the second-harmonic generation (SHG) response of amphiphilic push-pull chromophores to a transmembrane electric field. Our technique is based on the application of an alternating field across labeled giant unilamelar vesicles. For chromophore responses that are purely electro-optic, our technique provides an estimate of photoinduced charge shifts based on the observed dispersion of the field response, in accord with a two-level perturbation theory. These… CONTINUE READING