Electrical properties and fusion dynamics of in vitro membrane vesicles derived from separate parts of the contractile vacuole complex of Paramecium multimicronucleatum.

@article{Sugino2005ElectricalPA,
  title={Electrical properties and fusion dynamics of in vitro membrane vesicles derived from separate parts of the contractile vacuole complex of Paramecium multimicronucleatum.},
  author={Kazuyuki Sugino and Takashi Tominaga and Richard D M Allen and Yutaka Naitoh},
  journal={The Journal of experimental biology},
  year={2005},
  volume={208 Pt 20},
  pages={3957-69}
}
The contractile vacuole complex of Paramecium multimicronucleatum transforms into membrane-bound vesicles on excision from the cell. The I-V relationship was linear in a voltage range of -80 to +80 mV in all vesicles, despite being derived from different parts of the contractile vacuole complex. No voltage-gated unit currents were observed in membrane patches from the vesicles. Vesicles derived from the radial arm showed a membrane potential of >10 mV, positive with reference to the cytosol… CONTINUE READING
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