Electrical detection of germination of viable model Bacillus anthracis spores in microfluidic biochips.

@article{Liu2007ElectricalDO,
  title={Electrical detection of germination of viable model Bacillus anthracis spores in microfluidic biochips.},
  author={Yi-shao Liu and Thomas M Walter and Woo-Jin Chang and Kwan-Seop Lim and Liju Yang and Sang Woo Lee and Arthur I. Aronson and Rashid Bashir},
  journal={Lab on a chip},
  year={2007},
  volume={7 5},
  pages={603-10}
}
In this paper, we present a new impedance-based method to detect viable spores by electrically detecting their germination in real time within microfluidic biochips. We used Bacillus anthracis Sterne spores as the model organism. During germination, the spores release polar and ionic chemicals, such as dipicolinic acid (DPA), calcium ions, phosphate ions, and amino acids, which correspondingly increase the electrical conductivity of the medium in which the spores are suspended. We first present… CONTINUE READING