Electric shock-mediated transfection of cells. Characterization and optimization of electrical parameters.

@article{Winterbourne1988ElectricST,
  title={Electric shock-mediated transfection of cells. Characterization and optimization of electrical parameters.},
  author={D. J. Winterbourne and Sunil Thomas and John Hermon-Taylor and Ishrut Hussain and Alan P. Johnstone},
  journal={The Biochemical journal},
  year={1988},
  volume={251 2},
  pages={427-34}
}
The effect of various parameters on the electric shock-mediated permeabilization and transfection of CHO cells has been investigated. Up to 70% of the cells can be maintained transiently permeable to erythrosin B for periods of at least 1 h at 20 degrees C. Electrical conditions optimal for transient permeabilization were also optimal for efficient DNA transfection by pSV2neo. However, the DNA must be present during exposure to the electric field for efficient transformation. The same… CONTINUE READING