Electric field mediated loading of macromolecules in intact yeast cells is critically controlled at the wall level.

@article{Ganeva1995ElectricFM,
  title={Electric field mediated loading of macromolecules in intact yeast cells is critically controlled at the wall level.},
  author={Valentina Ganeva and Bojidar Galutzov and Justin Teissi{\'e}},
  journal={Biochimica et biophysica acta},
  year={1995},
  volume={1240 2},
  pages={229-36}
}
The mechanism of electric field mediated macromolecule transfer inside an intact yeast cell was investigated by observing, under a microscope, the fluorescence associated to cells after pulsation in a buffer containing two different hydrophilic fluorescent dyes. In the case of a small probe such as propidium iodide, a long lived permeabilized state was induced by the field as classically observed on wall free systems. Penetration of a 70 kDa FITC dextran was obtained only by using drastic… CONTINUE READING
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