Elaboration of an electroporation protocol for Bacillus cereus ATCC 14579.

  title={Elaboration of an electroporation protocol for Bacillus cereus ATCC 14579.},
  author={Nathalie Turgeon and Christian Laflamme and Jim Ho and Caroline Duchaine},
  journal={Journal of microbiological methods},
  volume={67 3},
An electro-transformation procedure was established for Bacillus cereus ATCC 14579. Using early growth-stage culture and high electric field, the ectroporation efficiency was up to 2 x 10(9) cfu microg(-1) ml(-1) with pC194 plasmid DNA. The procedure was tested with three other plasmids, of various sizes, replication mechanisms and selection markers, and the transformation efficiencies ranged between 2 x 10(6) and 1 x 10(8) cfu microg(-1) ml(-)(1). The effects of two wall-weakening agents on… CONTINUE READING

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